结论


本研究的结果表明，当存在一个以上的电子受体时，生物膜中的呼吸和代谢生理分层情况。 虽然苋菜红还原与阳极竞争电子，但脱色链球菌菌株S12同时与阳极和苋菜红呼吸。 此外，MFCs阳极室中出现了空间分离的呼吸模式。 浮游细胞和外层生物膜细胞倾向于使用苋菜红作为电子受体，而内层生物膜细胞倾向于使用阳极作为电子受体。 与仅用阳极呼吸的生物膜相比，额外的苋菜红呼吸驱散了生物膜中的质子积累。 阳极呼吸生物膜的氧化还原电位呈现先降低后升高的趋势，这与其介质占主导地位的性质相一致。 额外的苋菜呼吸对生物膜电位影响较小，但阳极电位显着降低。 与使用唯一电子受体呼吸的生物膜相比，由于生物膜内的双向呼吸电子转移，同时使用苋菜红和阳极呼吸的生物膜中观察到更高和更均匀的活性分布。 虽然苋菜红还原与阳极竞争电子，但脱色链球菌菌株S12同时与阳极和苋菜红呼吸。此外，MFCs阳极室中出现了空间分离的呼吸模式。浮游细胞和外层生物膜细胞倾向于使用苋菜红作为电子受体，而内层生物膜细胞倾向于使用阳极作为电子受体。与仅用阳极呼吸的生物膜相比，额外的苋菜红呼吸驱散了生物膜中的质子积累。阳极呼吸生物膜的氧化还原电位呈现先降低后升高的趋势，这与其介质占主导地位的性质相一致。额外的苋菜呼吸对生物膜电位影响较小，但阳极电位显著降低。与使用唯一电子受体呼吸的生物膜相比，由于生物膜内的双向呼吸电子转移，同时使用苋菜红和阳极呼吸的生物膜中观察到更高和更均匀的活性分布。


关联内容


支持信息


图S1 − S7。 此材料可通过互联网免费获取，网址为 http://pubs.acs.org.


作者信息


通讯作者


*电话：+862087684471。 传真：+862087684587。 电邮： xumy@gdim.cn.


作者贡献


Y.Y.，M.X.和G.S.设计了实验。 Y.Y.和Y.X.进行了实验。 Y.Y.，W.-M.W.，和M.X.分析数据并撰写手稿。


笔记


作者声明没有相互竞争的经济利益。


致谢


我们感谢Joy D.Van Nostrand博士对语言修订的热情帮助。 本研究得到中国国家基础研究计划（973计划）（2012CB22307）、中国广东自然科学基金（S2013010014596）、国家自然科学基金（51422803, 31200096）、广东科学院优秀学者课题（RCJJ201502）的资助。 广东省海洋经济区域创新发展示范项目（GD2012-D01-002）。


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生物膜的呼吸系统和生理层次中的电子受体的依赖性——摘要、介绍

生物膜的呼吸系统和生理层次中的电子受体的依赖性——材料和方法

生物膜的呼吸系统和生理层次中的电子受体的依赖性——结果和讨论

生物膜的呼吸系统和生理层次中的电子受体的依赖性——结论、致谢！